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Next is Agni Mahaboota after Vayu Mahabhoota, which has the vision (Roopa) as its sense attribute and warmth (Ushnatwa) as its main property. This helps in viewing the creation in life. In living beings Agni Mahabhoota is the major constituent of biological humor Pitta dosha. Agni provides the energy necessary for all activities in our body and can be utilized by our cells. As long as this Agni is proper, all the activities in body are carried out smoothly. Any disturbance in this Agni causes imbalance in the homeostasis (equilibrium) and disturbs physiology.

Entire Ayurvedic therapeutics is based on this concept of Agni. The branch of Ayurved which deals with internal medicine and treatment is called "Kayachikitsa", where Kaya means Agni and Chikitsa means treatment.